abstract：:Contact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or Rho...

journal_title：FEBS letters

authors： Anear E,Parish RW

更新日期：2012-05-07 00:00:00

abstract：:ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA...

journal_title：FEBS letters

authors： Li Z,Liu D

更新日期：2012-05-07 00:00:00

abstract：:Hepatitis C virus (HCV) often establishes a persistent infection that leads to chronic liver diseases. The viral core protein modulates various cellular activities involved in this process. We found two mutations, K23E and V31A, in the core gene of the transfected HCV JFH-1 genome, which had been replicated for a prol...

journal_title：FEBS letters

authors： Kang JI,Kwon YC,Ahn BY

更新日期：2012-05-07 00:00:00

abstract：:Transient receptor potential (TRP) proteins are sensory-related cation channels. TRPV subfamily responds to vanilloids, generating a Ca(2+) current. TRPV1, a thermal-sensitive non-selective ion channel, possesses six transmembrane helices and the intracellular N- and C-terminal domains. The latter contains the PIP(2) ...

journal_title：FEBS letters

authors： Aguado-Llera D,Bacarizo J,Gregorio-Teruel L,Taberner FJ,Cámara-Artigas A,Neira JL

更新日期：2012-04-24 00:00:00

abstract：:Y RNAs are approximately 100 nucleotide long conserved cytoplasmic non-coding RNAs, which produce smaller RNA fragments during apoptosis. Here we show that these smaller RNA molecules are also produced in non-stressed cells and in a range of human cancerous and non-cancerous cell types. Recent reports have speculated ...

journal_title：FEBS letters

authors： Nicolas FE,Hall AE,Csorba T,Turnbull C,Dalmay T

更新日期：2012-04-24 00:00:00

abstract：:RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...

journal_title：FEBS letters

authors： Niu Z,Jin W,Zhang L,Li X

更新日期：2012-04-05 00:00:00

abstract：:In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phos...

journal_title：FEBS letters

authors： Wu L,Li H,Jia CY,Cheng W,Yu M,Peng M,Zhu Y,Zhao Q,Dong YW,Shao K,Wu A,Wu XZ

更新日期：2012-04-05 00:00:00

abstract：:Ycf1p function is regulated by casein kinase 2α, Cka1p, via phosphorylation of Ser251. Cka1p-mediated phosphorylation of Ycf1p is attenuated in response to high salt stress. Previous results from our lab suggest a role for Ycf1p in cellular resistance to salt stress. Here, we show that Ycf1p plays an important role in...

journal_title：FEBS letters

authors： Paumi CM,Pickin KA,Jarrar R,Herren CK,Cowley ST

更新日期：2012-03-23 00:00:00

abstract：:Adiponectin is increasingly recognized as a potential therapeutic agent for the treatment of diabetes and other metabolic diseases. It circulates in plasma as homotrimers and higher-order oliogomers of homotrimers. To facilitate the production of active recombinant adiponectin as a therapeutic tool, we designed a sing...

journal_title：FEBS letters

authors： Min X,Lemon B,Tang J,Liu Q,Zhang R,Walker N,Li Y,Wang Z

更新日期：2012-03-23 00:00:00

abstract：:TLR3 and TLR4 utilize adaptor TRIF to activate interferon regulatory factor 3 (IRF3), resulting in interferon β (IFN-β) production to mediate anti-viral infection. In this report, we analyzed the effect of two known phosphatidylinositol 3-kinase (PI3K) inhibitors LY294002 and wortmannin on LPS- and poly(I:C)-induced I...

journal_title：FEBS letters

authors： Zhao W,Qi J,Wang L,Zhang M,Wang P,Gao C

更新日期：2012-03-23 00:00:00

abstract：:The transmembrane protein MIG-13 is a key regulator required for anterior migration of neural cells in Caenorhabditis elegans, but the signaling mechanisms involved remain unknown. Here, we isolated a suppressor mutation in the unc-71/adm-1 gene, which rescued the AVM neuron migration defect in mig-13 mutants. Genetic...

journal_title：FEBS letters

authors： Masuda H,Nakamura K,Takata N,Itoh B,Hirose T,Moribe H,Mekada E,Okada M

更新日期：2012-03-23 00:00:00

abstract：:Redox reactions of tyrosine play key roles in many biological processes, including water oxidation and DNA synthesis. We first review the redox properties of tyrosine (and other phenols) in small molecules and related polypeptides, then report work on (H20)/(Y48)-modified Pseudomonas aeruginosa azurin. The crystal str...

journal_title：FEBS letters

authors： Warren JJ,Winkler JR,Gray HB

更新日期：2012-03-09 00:00:00

abstract：:Determination of the protonation state of enzymes is a challenging problem in computational biophysics largely due to the vast number of possible protonic configurations. The protonation state dynamics of respiratory complex I was investigated via Monte Carlo and asynchronous dynamics simulations and a novel eigenvect...

journal_title：FEBS letters

authors： Couch V,Stuchebrukhov A

更新日期：2012-03-09 00:00:00

abstract：:This article presents an overview of the simulation studies of the behaviour of multihaem cytochromes using theoretical/computational methodologies, with an emphasis on cytochrome c(3). It starts with the first studies using rigid molecules and continuum electrostatic models, where protonation and redox events were tr...

journal_title：FEBS letters

authors： Soares CM,Baptista AM

更新日期：2012-03-09 00:00:00

abstract：:Upon dimerization by oxidation, Hsp33 functions as a molecular chaperone in prokaryotes. Previously published structures of both the inactive and active species are of doubtful relevance to the solution conformations since the inactive (reduced) crystal structure was dimeric, while the active (oxidized) species was cr...

journal_title：FEBS letters

authors： Lee YS,Ryu KS,Kim SJ,Ko HS,Sim DW,Jeon YH,Kim EH,Choi WS,Won HS

更新日期：2012-02-17 00:00:00

abstract：:The crystal structure for cce_0566 (171 aa, 19.4 kDa), a DUF269 annotated protein from the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142, was determined to 1.60Å resolution. Cce_0566 is a homodimer with each molecule composed of eight α-helices folded on one side of a three strand anti-parallel β-sheet. Hydrop...

journal_title：FEBS letters

authors： Buchko GW,Robinson H

更新日期：2012-02-17 00:00:00

abstract：:Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microar...

journal_title：FEBS letters

authors： Hu SN,Yu H,Zhang YB,Wu ZL,Yan YC,Li YX,Li YY,Li YP

更新日期：2012-02-03 00:00:00

abstract：:Friedreich ataxia is the most common recessive neurodegenerative disease and is caused by reduced expression of mitochondrial frataxin. Frataxin depletion causes impairment in iron-sulfur cluster and heme biosynthesis, disruption of iron homeostasis and hypersensitivity to oxidants. Currently no pharmacological treatm...

journal_title：FEBS letters

authors： Lefevre S,Sliwa D,Auchère F,Brossas C,Ruckenstuhl C,Boggetto N,Lesuisse E,Madeo F,Camadro JM,Santos R

更新日期：2012-01-20 00:00:00

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:Abstract Hyperhomocysteinemia has recently been identified as an important risk factor for Alzheimer's disease (AD). One of the potential mechanisms underlying harmful effects of homocysteine (Hcy) is site-specific acylation of proteins at lysine residues by homocysteine thiolactone (HCTL). The accumulation of amyloid...

journal_title：FEBS letters

authors： Khodadadi S,Riazi GH,Ahmadian S,Hoveizi E,Karima O,Aryapour H

更新日期：2012-01-20 00:00:00

abstract：:Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant p...

journal_title：FEBS letters

authors： Hannauer M,Schäfer M,Hoegy F,Gizzi P,Wehrung P,Mislin GL,Budzikiewicz H,Schalk IJ

更新日期：2012-01-02 00:00:00

abstract：:Several neurodegenerative disorders are characterized by the accumulation of proteinaceous inclusions in the central nervous system. These inclusions are frequently composed of a mixture of aggregation-prone proteins. Here, we used a bimolecular fluorescence complementation assay to study the initial steps of the co-a...

journal_title：FEBS letters

authors： Herrera F,Outeiro TF

更新日期：2012-01-02 00:00:00

abstract：:Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production afte...

journal_title：FEBS letters

authors： Nabben M,Hoeks J,Moonen-Kornips E,van Beurden D,Briedé JJ,Hesselink MK,Glatz JF,Schrauwen P

更新日期：2011-12-15 00:00:00

abstract：:Regulatory T-cells (Tregs) are the guardians of peripheral tolerance acting to prevent autoimmune diseases such as systemic lupus erythomatosus (SLE) and rheumatoid arthritis (RA). Defects in Tregs have been reported in these two diseases despite significant differences in their clinical phenotype and pathogenesis. In...

journal_title：FEBS letters

authors： Chavele KM,Ehrenstein MR

更新日期：2011-12-01 00:00:00

abstract：:Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system characterized by widespread inflammation, focal demyelination and a variable degree of axonal and neuronal loss. Ionic conductances regulate T cell activation as well as neuronal function and thus have been found to play a crucial ...

journal_title：FEBS letters

authors： Ehling P,Bittner S,Budde T,Wiendl H,Meuth SG

更新日期：2011-12-01 00:00:00

abstract：:The nervous and immune systems have similar functional characteristics. Both have an intricate network of synaptic connections and an exquisite communication system that enable intercellular signal transduction. Although semaphorins were originally identified as guidance cues in neural development, accumulating eviden...

journal_title：FEBS letters

authors： Okuno T,Nakatsuji Y,Kumanogoh A

更新日期：2011-12-01 00:00:00

abstract：:We previously reported that IκBL prevents experimental autoimmune arthritis. The molecular mechanism, however, still remains unclear. In contrast to four splicing-isoforms of IκBL in human, two isoforms were identified in mouse. The major isoform IκBL-α(S) suppressed LPS-induced NF-κB activation and transcription of T...

journal_title：FEBS letters

authors： Chiba T,Miyashita K,Sugoh T,Warita T,Inoko H,Kimura M,Sato T

更新日期：2011-11-16 00:00:00

abstract：:Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here...

journal_title：FEBS letters

authors： Qiu F,Wang J,Spray DC,Scemes E,Dahl G

更新日期：2011-11-04 00:00:00

abstract：:Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...

journal_title：FEBS letters

authors： Jin H,Yang X,Liu K,Gu Q,Xu X

更新日期：2011-11-04 00:00:00

abstract：:USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...

journal_title：FEBS letters

authors： Elliott PR,Liu H,Pastok MW,Grossmann GJ,Rigden DJ,Clague MJ,Urbé S,Barsukov IL

更新日期：2011-11-04 00:00:00

abstract：:The sialic acid of complex N-glycans can be biochemically engineered by substituting the physiological precursor N-acetylmannosamine with non-natural N-acylmannosamines. The Kv3.1 glycoprotein, a neuronal voltage-gated potassium channel, contains sialic acid. Western blots of the Kv3.1 glycoprotein isolated from trans...

journal_title：FEBS letters

authors： Hall MK,Reutter W,Lindhorst T,Schwalbe RA

更新日期：2011-10-20 00:00:00

abstract：:HBV particles with genome derived from spliced mRNAs accumulate in patients with virus-derived severe liver necrosis and fibrosis. We investigated the role of an intronic element (intronic splicing silencer-long, ISS(L)) on splicing of HBV minigene transcripts. Removal of the entire ISS(L) showed two-fold increase in ...

journal_title：FEBS letters

authors： Chowdhury JB,Roy D,Ghosh S

更新日期：2011-10-20 00:00:00

abstract：:An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...

journal_title：FEBS letters

authors： Alkhatib SG,Landry JW

更新日期：2011-10-20 00:00:00

abstract：:Hell's Gate globin I (HGbI), a heme-containing protein structurally homologous to mammalian neuroglobins, has been identified from an acidophilic and thermophilic obligate methanotroph, Methylacidiphilum infernorum. HGbI has very high affinity for O(2) and shows barely detectable autoxidation in the pH range of 5.2-8....

journal_title：FEBS letters

authors： Teh AH,Saito JA,Baharuddin A,Tuckerman JR,Newhouse JS,Kanbe M,Newhouse EI,Rahim RA,Favier F,Didierjean C,Sousa EH,Stott MB,Dunfield PF,Gonzalez G,Gilles-Gonzalez MA,Najimudin N,Alam M

更新日期：2011-10-20 00:00:00

abstract：:Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we...

journal_title：FEBS letters

authors： Naganuma T,Sato Y,Sassa T,Ohno Y,Kihara A

更新日期：2011-10-20 00:00:00

abstract：:Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that ...

journal_title：FEBS letters

authors： Kim HL,Park MB,Park YS

更新日期：2011-10-03 00:00:00

abstract：:MiR-140 is a microRNA specially involved in chondrogenesis and osteoarthritis pathogenesis. However, its transcriptional regulation and target genes in cartilage development are not fully understood. Here we detected that miR-140 was uniquely expressed in chondrocyte and suppressed by Wnt/β-catenin signalling. The miR...

journal_title：FEBS letters

authors： Yang J,Qin S,Yi C,Ma G,Zhu H,Zhou W,Xiong Y,Zhu X,Wang Y,He L,Guo X

更新日期：2011-10-03 00:00:00

abstract：:AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 tran...

journal_title：FEBS letters

authors： Zhang W,Xu Y,Chen Z,Xu Z,Xu H

更新日期：2011-10-03 00:00:00

abstract：:Sphingolipids are implied in several regulatory processes, including cell death. Levels of the free sphingobase t18:0 (phytosphingosine) increase in Arabidopsis in response to the bacterial pathogen Pseudomonas syringae. To gain information on sphingobase-induced signaling, we determined kinetics of leaf reactive oxyg...

journal_title：FEBS letters

authors： Peer M,Bach M,Mueller MJ,Waller F

更新日期：2011-10-03 00:00:00

abstract：:The COP9 signalosome (CSN) is a platform for protein communication in eukaryotic cells. It has an intrinsic metalloprotease that removes the ubiquitin (Ub)-like protein Nedd8 from cullins. CSN-mediated deneddylation regulates culling-RING Ub ligases (CRLs) and controls ubiquitination of proteins involved in DNA damage...

journal_title：FEBS letters

authors： Hannss R,Dubiel W

更新日期：2011-09-16 00:00:00